We offer selective cleavage of the antibody into Fab fragments using papain (refer to the figure below). Fc fragment and non-cleaved antibodies are removed using Protein A columns.
There are several advantages of using antibody fragments:
- Reduction of nonspecific Fc-binding to the cells and elimination of Fc-associated effector functions
- Better performances in in vivo experiments due to lower immunogenicity
- Enhanced penetration of tissue sections
- Proven tool for assisting crystallization of proteins